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Citation Information
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 15;945-946:53-9. doi: 10.1016/j.jchromb.2013.11.038. Epub 2013 Nov 25.
Investigation of volatile organic metabolites in lung cancer pleural effusions by solid-phase microextraction and gas chromatography/mass spectrometry.
Liu H, Wang H, Li C, Wang L, Pan Z, Wang L.
Analytical methods | ||
|---|---|---|
| Comparative study | #1 | |
| Chromatography | GC | |
| Ion source | EI | |
| Positive/Negative mode | positive | |
| Mass analyzer | single quad | |
| Identification level | – | |
Sample information | ||
|---|---|---|
| Comparative study | #1 | |
| Country | China | |
| Specimen | pleural effusion | |
| Marker function | diagnosis | |
| Participants(Case) | Cancer type | NSCLC, SCLC |
| Stage | – | |
| Number | 20 | |
| Gender (M,F) | 16, 4 | |
| Mean age (range) (M,F) | 73 (53-85) | |
| Smoking status | – | |
| Participants(Control) | Type | tuberculous pleurisy, pneumonia |
| Number | 20 | |
| Gender (M,F) | 14, 6 | |
| Mean age (range) (M,F) | 63.6 (44-86) | |
| Smoking status | – | |
Data processing and metabolite identification | |
|---|---|
| Data processing software | – |
| Database search | NIST02 library |
Statistical analysis | |
|---|---|
| Differential analysis method | Student's t-test, corrected t-test |
| Classification method | – |
| Survival analysis method | – |
Lung cancer-related metabolites identified in the paper
| Metabolite | Author-emphasized biomarkers | Mean concentration (case) | Mean concentration (control) | Fold change (case/control) | P-value | FDR | VIP |
|---|---|---|---|---|---|---|---|
| 2-ethyl-1-hexanol | – | 1373900 ± 1112200 | 65000 ± 55500 | 21.10 | 1.00e-02 | – | – |
| longifolene | – | 1500 ± 1400 | 700 ± 500 | 2.20 | 0.04 | – | – |
| 2-phenyl-2-propanol | – | 26300 ± 26100 | 1800 ± 1100 | 14.00 | 0.03 | – | – |
| cyclohexanone | – | 2800700 ± 2566000 | 158700 ± 149200 | 17.50 | 3.00e-03 | – | – |
| 1,2,4,5-tetramethylbenzene | – | 50200 ± 50300 | 6500 ± 5400 | 7.70 | 5.00e-03 | – | – |
| Metabolite | Author-emphasized biomarkers | Cutoff value | AUROC (95%CI) | Sensitivity (%) | Specificity (%) | Accuracy (%) |
|---|---|---|---|---|---|---|
| 2-ethyl-1-hexanol | – | – | – | – | – | – |
| longifolene | – | – | – | – | – | – |
| 2-phenyl-2-propanol | – | – | – | – | – | – |
| cyclohexanone | – | – | – | – | – | – |
| 1,2,4,5-tetramethylbenzene | – | – | – | – | – | – |
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