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Citation Information
J Proteome Res. 2014 Sep 5;13(9):4040-6. doi: 10.1021/pr5003774. Epub 2014 Aug 21.
Untargeted mass spectrometry-based metabolomic profiling of pleural effusions: fatty acids as novel cancer biomarkers for malignant pleural effusions.
Lam CW, Law CY.
Analytical methods | ||
|---|---|---|
| Comparative study | #1 | |
| Chromatography | LC | |
| Ion source | ESI | |
| Positive/Negative mode | both | |
| Mass analyzer | TripleTOF | |
| Identification level | MS/MS | |
Sample information | ||
|---|---|---|
| Comparative study | #1 | |
| Country | China | |
| Specimen | pleural effusion | |
| Marker function | diagnosis | |
| Participants(Case) | Cancer type | NSCLC, SCLC, anaplastic carcinoma |
| Stage | – | |
| Number | 32 | |
| Gender (M,F) | 13, 19 | |
| Mean age (range) (M,F) | 72.8 ± 11.4 | |
| Smoking status | smoker, non-smoker | |
| Participants(Control) | Type | pulmonary tuberculosis |
| Number | 18 | |
| Gender (M,F) | 10, 8 | |
| Mean age (range) (M,F) | 59.7 ± 25.2 | |
| Smoking status | smoker, non-smoker | |
Data processing and metabolite identification | |
|---|---|
| Data processing software | PeakView, LipidView (AB SCIEX), XCMS |
| Database search | HMDB |
Statistical analysis | |
|---|---|
| Differential analysis method | t-test, OPLS-DA |
| Classification method | ROC curve analysis |
| Survival analysis method | – |
Lung cancer-related metabolites identified in the paper
| Metabolite | Author-emphasized biomarkers | Mean concentration (case) | Mean concentration (control) | Fold change (case/control) | P-value | FDR | VIP |
|---|---|---|---|---|---|---|---|
| ceramide | – | – | – | – | 1.72e-05 | – | – |
| oleic acid | – | – | – | – | 8.23e-08 | – | – |
| Metabolite | Author-emphasized biomarkers | Cutoff value | AUROC (95%CI) | Sensitivity (%) | Specificity (%) | Accuracy (%) |
|---|---|---|---|---|---|---|
| ceramide | – | – | 0.726–0.938 | 72.2 | 90.6 | – |
| oleic acid | – | – | 0.866–0.996 | 84.4 | 100 | – |
Paper graphical summary
