Showing paper detailed information
Citation Information
Biomed Res Int. 2015;2015:183624. doi: 10.1155/2015/183624. Epub 2015 Apr 16.
Biomarker identification and pathway analysis by serum metabolomics of lung cancer.
Chen Y, Ma Z, Min L, Li H, Wang B, Zhong J, Dai L.
Analytical methods | |||||
|---|---|---|---|---|---|
| Comparative study | #1 | #2 | #3 | #4 | |
| Chromatography | LC | LC | GC | GC | |
| Ion source | ESI | ESI | – | – | |
| Positive/Negative mode | positive | positive | – | – | |
| Mass analyzer | Q-TOF | Q-TOF | – | – | |
| Identification level | – | – | – | – | |
Sample information | |||||
|---|---|---|---|---|---|
| Comparative study | #1 | #2 | #3 | #4 | |
| Country | China | China | China | China | |
| Specimen | serum | serum | serum | serum | |
| Marker function | diagnosis | diagnosis | diagnosis | diagnosis | |
| Participants(Case) | Cancer type | adenocarcinoma, squamous cell carcinoma, large cell carcinoma | adenocarcinoma, squamous cell carcinoma, large cell carcinoma | adenocarcinoma, squamous cell carcinoma, large cell carcinoma | adenocarcinoma, squamous cell carcinoma, large cell carcinoma |
| Stage | I, II, III | I, II, III | I, II, III | I, II, III | |
| Number | 30 | 30 | 30 | 30 | |
| Gender (M,F) | 9, 21 | 9, 21 | 9, 21 | 9, 21 | |
| Mean age (range) (M,F) | 61.58 ± 10.67 | 61.58 ± 10.67 | 61.58 ± 10.67 | 61.58 ± 10.67 | |
| Smoking status | – | – | – | – | |
| Participants(Control) | Type | healthy | before vs. after treatment (operation) | healthy | before vs. after treatment (operation) |
| Number | 30 | 30 | 30 | 30 | |
| Gender (M,F) | 11, 19 | 9, 21 | 11, 19 | 9, 21 | |
| Mean age (range) (M,F) | 60.35 ± 12.48 | 61.58 ± 10.67 | 60.35 ± 12.48 | 61.58 ± 10.67 | |
| Smoking status | – | – | – | – | |
Data processing and metabolite identification | |
|---|---|
| Data processing software | Mass Hunter Qualitative Analysis Software (Agilent Technologies) |
| Database search | METLIN |
Statistical analysis | |
|---|---|
| Differential analysis method | independent t-test |
| Classification method | – |
| Survival analysis method | – |
Lung cancer-related metabolites identified in the paper
| Metabolite | Author-emphasized biomarkers | Mean concentration (case) | Mean concentration (control) | Fold change (case/control) | P-value | FDR | VIP |
|---|---|---|---|---|---|---|---|
| serine | V | 114.92 ± 89.30 | 183.48 ± 96.63 | 0.63 | 1.00e-03 | – | – |
| serine | V | 114.92 ± 89.30 | 284.16 ± 184.76 | 0.40 | 1.00e-03 | – | – |
| γ-linolenic acid | V | 1245.99 ± 595.41 | 500.34 ± 204.80 | 2.49 | 1.00e-03 | – | – |
| γ-linolenic acid | V | 1245.99 ± 595.41 | 602.06 ± 226.28 | 2.07 | 1.00e-03 | – | – |
| phosphorylcholine | V | 133.28 ± 75.49 | 72.70 ± 14.16 | 1.83 | 1.00e-03 | – | – |
| phosphorylcholine | V | 133.28 ± 75.49 | 82.17 ± 28.31 | 1.62 | 1.00e-03 | – | – |
| 2,3,4-trihydroxybutyric acid | V | 24.63 ± 24.13 | 71.89 ± 30.60 | 0.34 | 1.00e-03 | – | – |
| 2,3,4-trihydroxybutyric acid | V | 24.63 ± 24.13 | 23.02 ± 14.47 | 1.07 | 1.00e-03 | – | – |
| 9,12-octadecadienoic acid | V | 24.90 ± 18.09 | 9.88 ± 5.79 | 2.52 | 1.00e-03 | – | – |
| 9,12-octadecadienoic acid | V | 24.90 ± 18.09 | 13.57 ± 9.30 | 1.83 | 1.00e-03 | – | – |
| glycerophospho-N-arachidonoyl ethanolamine | V | 2722.76 ± 769.63 | 1355.53 ± 282.89 | 2.01 | 1.00e-03 | – | – |
| glycerophospho-N-arachidonoyl ethanolamine | V | 2722.76 ± 769.63 | 1714.79 ± 399.47 | 1.59 | 1.00e-03 | – | – |
| sphingosine | V | 53.33 ± 35.95 | 139.60 ± 38.75 | 0.38 | 1.00e-03 | – | – |
| sphingosine | V | 53.33 ± 35.95 | 141.78 ± 42.42 | 0.38 | 1.00e-03 | – | – |
| α-hydroxyisobutyric acid | V | 556.54 ± 220.00 | 306.84 ± 153.64 | 1.81 | 1.00e-03 | – | – |
| α-hydroxyisobutyric acid | V | 556.54 ± 220.00 | 803.58 ± 329.41 | 0.69 | – | – | – |
| oleic acid | V | 605.66 ± 361.44 | 244.99 ± 131.32 | 2.47 | 1.00e-03 | – | – |
| oleic acid | V | 605.66 ± 361.44 | 346.58 ± 164.66 | 1.75 | 1.00e-03 | – | – |
| prasterone sulfate | V | 71.99 ± 38.72 | 106.80 ± 31.70 | 0.67 | 1.00e-03 | – | – |
| prasterone sulfate | V | 71.99 ± 38.72 | 50.93 ± 22.26 | 1.41 | – | – | – |
| Metabolite | Author-emphasized biomarkers | Cutoff value | AUROC (95%CI) | Sensitivity (%) | Specificity (%) | Accuracy (%) |
|---|---|---|---|---|---|---|
| serine | V | 113.21 | 0.774 (0.645-0.904) | 83.33 | 73.33 | – |
| serine | V | 130.97 | 0.825 (0.721) | 80 | 76.67 | – |
| γ-linolenic acid | V | 805.17 | 0.889 (0.806-0.972) | 76.67 | 93.33 | – |
| γ-linolenic acid | V | 808.24 | 0.847 (0.744–0.949) | 76.67 | 90 | – |
| phosphorylcholine | V | 78.32 | 0.874 (0.780-0.969) | 90 | 80 | – |
| phosphorylcholine | V | 84.69 | 0.781 (0.666–0.896) | 76.67 | 70 | – |
| 2,3,4-trihydroxybutyric acid | V | 45.87 | 0.88 (0.794-0.966) | 86.67 | 83.33 | – |
| 2,3,4-trihydroxybutyric acid | V | – | – | – | – | – |
| 9,12-octadecadienoic acid | V | 13.56 | 0.704 (0.562-0.847) | 66.67 | 83.33 | – |
| 9,12-octadecadienoic acid | V | 21.29 | 0.645 (0.499–0.791) | 53.33 | 86.67 | – |
| glycerophospho-N-arachidonoyl ethanolamine | V | 1752.6 | 0.983 (0.960-1.007) | 96.67 | 90 | – |
| glycerophospho-N-arachidonoyl ethanolamine | V | 1988.46 | 0.916 (0.847–0.984) | 76.67 | 93.33 | – |
| sphingosine | V | 102.76 | 0.957 (0.894-1.019) | 90 | 96.67 | – |
| sphingosine | V | 86.48 | 0.966 (0.911) | 96.67 | 90 | – |
| α-hydroxyisobutyric acid | V | 365.23 | 0.86 (0.764-0.956) | 80 | 80 | – |
| α-hydroxyisobutyric acid | V | – | – | – | – | – |
| oleic acid | V | 402.22 | 0.749 (0.614-0.884) | 70 | 86.67 | – |
| oleic acid | V | 489.02 | 0.694 (0.550–0.838) | 60 | 80 | – |
| prasterone sulfate | V | 91.07 | 0.787 (0.670-0.905) | 76.67 | 80 | – |
| prasterone sulfate | V | – | – | – | – | – |
Paper graphical summary
