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Biomed Chromatogr. 2016 Nov;30(11):1796-1806. doi: 10.1002/bmc.3755. Epub 2016 Jun 8.
Simultaneous determination of thirteen kinds of amino acid and eight kinds of acylcarnitine in human serum by LC-MS/MS and its application to measure the serum concentration of lung cancer patients.
Ni J,, Xu L, Li W, Wu L.
Analytical methods | ||
|---|---|---|
| Comparative study | #1 | |
| Chromatography | LC | |
| Ion source | ESI | |
| Positive/Negative mode | positive | |
| Mass analyzer | triple quadrupole | |
| Identification level | MS/MS | |
Sample information | ||
|---|---|---|
| Comparative study | #1 | |
| Country | China | |
| Specimen | serum | |
| Marker function | diagnosis | |
| Participants(Case) | Cancer type | lung cancer |
| Stage | – | |
| Number | 40 | |
| Gender (M,F) | 14, 26 | |
| Mean age (range) (M,F) | 67 | |
| Smoking status | – | |
| Participants(Control) | Type | healthy |
| Number | 100 | |
| Gender (M,F) | – | |
| Mean age (range) (M,F) | – | |
| Smoking status | – | |
Data processing and metabolite identification | |
|---|---|
| Data processing software | – |
| Database search | – |
Statistical analysis | |
|---|---|
| Differential analysis method | one-way ANOVA |
| Classification method | – |
| Survival analysis method | – |
Lung cancer-related metabolites identified in the paper
| Metabolite | Author-emphasized biomarkers | Mean concentration (case) | Mean concentration (control) | Fold change (case/control) | P-value | FDR | VIP |
|---|---|---|---|---|---|---|---|
| C14-carnitine | V | 0.04 ± 0.02 μmol/L | 0.02 ± 0.02 μmol/L | – | 1.00e-04 | – | – |
| C6-carnitine | V | 0.06 ± 0.09 μmol/L | 0.06 ± 0.06 μmol/L | – | 0.99 | – | – |
| C8-carnitine | V | 0.06 ± 0.11 μmol/L | 0.08 ± 0.09 μmol/L | – | 0.32 | – | – |
| C5-carnitine | V | 0.18 ± 0.09 μmol/L | 0.10 ± 0.04 μmol/L | – | 1.00e-04 | – | – |
| C16-carnitine | V | 0.38 ± 0.21 μmol/L | 0.19 ± 0.08 μmol/L | – | 1.00e-04 | – | – |
| C4-carnitine | V | 0.54 ± 0.40 μmol/L | 0.22 ± 0.10 μmol/L | – | 1.00e-04 | – | – |
| C3-carnitine | V | 1.230 ± 0.72 μmol/L | 0.58 ± 0.22 μmol/L | – | 1.00e-04 | – | – |
| tyrosine | V | 114.95 ± 35.17 μmol/L | 88.03 ± 16.80 μmol/L | – | 1.00e-04 | – | – |
| histidine | V | 122.48 ± 34.12 μmol/L | 96.47 ± 20.59 μmol/L | – | 1.00e-04 | – | – |
| leucine | V | 129.40 ± 35.12 μmol/L | 116.60 ± 23.82 μmol/L | – | 0.01 | – | – |
| valine | V | 136.60 ± 35.57 μmol/L | 165.62 ± 28.08 μmol/L | – | 1.00e-04 | – | – |
| alanine | V | 146.50 ± 53.53 μmol/L | 141.98 ± 32.66 μmol/L | – | 0.54 | – | – |
| ornithine | V | 197.29 ± 117.34 μmol/L | 152.54 ± 64.60 μmol/L | – | 4.60e-03 | – | – |
| methionine | V | 21.02 ± 6.66 μmol/L | 33.09 ± 6.38 μmol/L | – | 1.00e-04 | – | – |
| arginine | V | 224.94 ± 72.52 μmol/L | 123.76 ± 43.14 μmol/L | – | 1.00e-04 | – | – |
| citrulline | V | 23.41 ± 10.00 μmol/L | 42.14 ± 12.77 μmol/L | – | 1.00e-04 | – | – |
| aspartate | V | 32.90 ± 11.01 μmol/L | 48.33 ± 27.02 μmol/L | – | 6.00e-04 | – | – |
| glycine | V | 408.80 ± 107.40 μmol/L | 420.01 ± 74.52 μmol/L | – | 0.48 | – | – |
| glutamate | V | 76.50 ± 21.56 μmol/L | 87.55 ± 27.25 μmol/L | – | 0.02 | – | – |
| C2-carnitine | V | 8.52 ± 4.70 μmol/L | 8.76 ± 2.94 μmol/L | – | 0.71 | – | – |
| phenylalanine | V | 97.25 ± 31.82 μmol/L | 68.00 ± 13.33 μmol/L | – | 1.00e-04 | – | – |
| Metabolite | Author-emphasized biomarkers | Cutoff value | AUROC (95%CI) | Sensitivity (%) | Specificity (%) | Accuracy (%) |
|---|---|---|---|---|---|---|
| C14-carnitine | V | – | – | – | – | – |
| C6-carnitine | V | – | – | – | – | – |
| C8-carnitine | V | – | – | – | – | – |
| C5-carnitine | V | – | – | – | – | – |
| C16-carnitine | V | – | – | – | – | – |
| C4-carnitine | V | – | – | – | – | – |
| C3-carnitine | V | – | – | – | – | – |
| tyrosine | V | – | – | – | – | – |
| histidine | V | – | – | – | – | – |
| leucine | V | – | – | – | – | – |
| valine | V | – | – | – | – | – |
| alanine | V | – | – | – | – | – |
| ornithine | V | – | – | – | – | – |
| methionine | V | – | – | – | – | – |
| arginine | V | – | – | – | – | – |
| citrulline | V | – | – | – | – | – |
| aspartate | V | – | – | – | – | – |
| glycine | V | – | – | – | – | – |
| glutamate | V | – | – | – | – | – |
| C2-carnitine | V | – | – | – | – | – |
| phenylalanine | V | – | – | – | – | – |
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